Journal: bioRxiv

Article Title: Fat1 deletion enhances Fibro-Adipogenic Differentiation and Adipogenic expansion following injury in skeletal muscle

doi: 10.64898/2026.04.21.719880

Figure Lengend Snippet: Fat1 LacZ induction by Cardiotoxin (CTX), 5 days after injury . (A) Scheme of the experiment, in which adult Fat1 LacZ/+ mice were injured by injection of CTX in the Tibialis anterior muscle (TA), and showing the timeline, with injury occurring at day 0, and muscle collection for histological analyses at 5 days post-injury. (B) Fat1 LacZ expression is visualized on the same muscle cross-sections as in of a Fat1 LacZ/+ mouse 5 days after CTX injury in the TA, with unijected (left) and injected (right) sides, by Salmon gal staining (top), or immunohistochemistry with anti-β-galactosidase (green) and laminin (white) antibodies (with DAPI, blue), showing that expression is undetectable in the uninjured adult muscle, but induced by the CTX injury. (C, D, E) high magnification images at the level of the actively regenerating area of the lesion, to show at different magnifications the combination of anti-β-galactosidase (green) and laminin (white) with Pax7 (red, C), Pdgfra (red, D, and iba1 (red, E). Scalebars: (B) 500 µm; (C) top images: 20 µm, bottom images 50 µm, (D,E) 50 µm.

Article Snippet: Purified rat anti-mouse CD104a (Pdgfra) monoclonal antibody, clone APA5, BD Biosciences #558774, RRID: AB_397117; XP Rabbit anti-Perilipin monoclonal antibody, clone D1D8; Cell signaling technology, #9349, RRID: AB_10829911; Chicken anti-GFP, Aves, #GFP-1020, RRID: AB_10000240; Rat monoclonal anti mouse Tenascin-C (TnC) antibody, clone Mtn-12, Thermofisher Scientific, #MA1-26778, RRID: AB_2256026; Mouse monoclonal anti-Chicken PAX7, IgG1, Developmental studies hybridoma Bank, #Pax7, RRID: AB_528428; Rabbit anti-Iba1 polyclonal antibody, Wako Chemicals, #019-19741, RRID: AB_839504; Alexa-Fluor-conjugated Phalloidin, Thermofisher Scientific, Alexa-Fluor-488: A12379; Alexa-Fluor-546: A22283; Alexa-Fluor-647: A22287.

Techniques: Injection, Expressing, Staining, Immunohistochemistry

Journal: The Journal of Poultry Science

Article Title: Involvement of Fibro-Adipogenic Progenitors and Cellular Communication Network Family Signaling in the Impaired Muscle Regeneration of Wooden Breast

doi: 10.2141/jpsa.2026009

Figure Lengend Snippet: Immunofluorescence analysis across three stages showing PAX7 protein (myosatellite cell; a, d, g) and PDGFRα protein (FAP; b, e, h). Figures a–c, d–f, and g–i show representative images of normal, mild, and severe WB, respectively. Images c, f, and i are overlays of a and b, d and e, and g and h, respectively. Arrows indicate areas of degeneration and regeneration. Arrowheads indicate fibrotic areas. Bars = 50 μm. FAP: fibro-adipogenic progenitor, PAX7: paired box 7, and PDGFRα: platelet-derived growth factor receptor alpha.

Article Snippet: After three washes with 0.01 M PBS, the sections were incubated overnight at 4°C with a mouse primary antibody against PAX7 (DSHB Hybridoma Product PAX7; deposited by A. Kawakami), diluted 1:100 (0.23 μg/mL), to detect PAX7.

Techniques: Immunofluorescence, Derivative Assay

Journal: The Journal of Poultry Science

Article Title: Involvement of Fibro-Adipogenic Progenitors and Cellular Communication Network Family Signaling in the Impaired Muscle Regeneration of Wooden Breast

doi: 10.2141/jpsa.2026009

Figure Lengend Snippet: Validation of gene expression by quantitative real-time PCR (qPCR). mRNA expression levels of PAX7 (a), MYOD (b), MYOG (c), PDGFRα (d), and CCN family members (e-h, CCN1 , CCN2 , CCN3 , and CCN4 , respectively) in normal, mild, and severe WB muscle samples (n = 37, 26, and 14, respectively). Relative expression was normalized to RPL30 and expressed relative to the mean of the normal group (set as 1). Data are presented as mean ± standard error. Asterisks indicate significant differences (* P < 0.05, and ** P < 0.005) as determined by one-way ANOVA with Tukey-Kramer HSD test for post-hoc comparisons.

Article Snippet: After three washes with 0.01 M PBS, the sections were incubated overnight at 4°C with a mouse primary antibody against PAX7 (DSHB Hybridoma Product PAX7; deposited by A. Kawakami), diluted 1:100 (0.23 μg/mL), to detect PAX7.

Techniques: Biomarker Discovery, Gene Expression, Real-time Polymerase Chain Reaction, Expressing

Journal: The Journal of Poultry Science

Article Title: Involvement of Fibro-Adipogenic Progenitors and Cellular Communication Network Family Signaling in the Impaired Muscle Regeneration of Wooden Breast

doi: 10.2141/jpsa.2026009

Figure Lengend Snippet: Correlation analysis of fibrosis/adipogenesis rate and gene expression levels. Heatmap showing Spearman’s rank correlation coefficients (Rho) between the fibrosis/adipogenesis rate and the expression levels of PAX7, MYOG, PDGFRα, CCN1, CCN2, CCN3, CCN4 , and MYOD . Color intensity represents the strength and direction of the correlation (blue = negative correlation, red = positive correlation and white = no correlation). Numerical values in each tile represent Rho.

Article Snippet: After three washes with 0.01 M PBS, the sections were incubated overnight at 4°C with a mouse primary antibody against PAX7 (DSHB Hybridoma Product PAX7; deposited by A. Kawakami), diluted 1:100 (0.23 μg/mL), to detect PAX7.

Techniques: Gene Expression, Expressing

Journal: Poultry Science

Article Title: Simultaneous improvement of breast muscle yield and meat quality in Langshan chickens

doi: 10.1016/j.psj.2026.106552

Figure Lengend Snippet: Comparation of myogenic capacity differences in Langshan chickens with different percentage of breast muscle yield. (A) Representative images of PAX7 and MYOD1 co-localization (scale bar = 50 μm). (B) Number of PAX7 + satellite cells (SCs) per unit area. (C) Number of MYOD1 + SCs per unit area. (D) Percentage of PAX7 + / MYOD1 + and PAX7 + /MYOD1 + cells among Pax7+ SCs. (E) Relative mRNA expression of myogenic regulatory factors. LPB: Low percentage of breast muscle yield group; HPB: High percentage of breast muscle yield group. Data were expressed as the mean ± SEM, n = 7. * P < 0.05, ** P < 0.01, *** P < 0.001.

Article Snippet: The primary antibodies used in the immunofluorescence experiment were as follows: mouse anti-PAX7 monoclonal antibody (DSHB, IA), rabbit anti-MYOD1 polyclonal antibody (Affinity Biosciences, Melbourne, Australia).

Techniques: Expressing

Journal: Poultry Science

Article Title: Simultaneous improvement of breast muscle yield and meat quality in Langshan chickens

doi: 10.1016/j.psj.2026.106552

Figure Lengend Snippet: Untargeted metabolomic analysis of breast muscle in Langshan chickens with different percentage of breast muscle yield. (A) Principal component analysis of samples from HPB and LPB groups. (B) Volcano plot showing differential metabolites. (C-D) Bubble plot showing the top 30 up-regulated or down-regulated differential metabolites in the HPB group. (E) Mulberry plot showing top 10 KEGG pathway enriched for up-regulated or down-regulated differential metabolites. (G) Correlation analysis of muscle fiber characteristics, and differential metabolites. (H) Relative mRNA expression of lipid metabolism–related genes. (I) Relative mRNA expression of proliferation-related genes ( PAX7, MYF5, PCNA, CCND1, and CDK2 ) after 24 h treatment of different concentrations of DPA in growth medium. (J) Representative morphological images of myotube formation following 48 h of differentiation with different concentrations of DPA treatment. (K) Relative mRNA expression of myogenic differentiation–related genes ( MYOD1, MYOG, MEF2C, and MHC ) after 48 h of differentiation. LPB: Low percentage of breast muscle yield group; HPB: High percentage of breast muscle yield group. Data were expressed as the mean ± SEM, n = 7. * P < 0.05, ** P < 0.01, *** P < 0.001.

Article Snippet: The primary antibodies used in the immunofluorescence experiment were as follows: mouse anti-PAX7 monoclonal antibody (DSHB, IA), rabbit anti-MYOD1 polyclonal antibody (Affinity Biosciences, Melbourne, Australia).

Techniques: Metabolomic, Expressing, Cell Characterization